FERTILITY BOOSTER

Microinjection techniques

     Microinjection fertilization is an important innovation emerged in recent afros, which finally result in a viable treatment even in the most difficult cases of male infertility. Previously the only option that could provide doctors was the adoption or insemination from donors. Now, new techniques such as ICSI microinjection offer genuine therapeutic solution. In the ICSI procedure the most powerful microscopes and instruments "microscopic manipulation" are used; embryologists> for example, can hold a single human egg on the tip of a fine suction pipette and penetrate with a thinner than the diameter of a hair needle seven or more times. Needle through a single sperm enters the egg cytoplasm. In most cases (60-70%) achieved sperm fertilize the egg three days later can be transferred to the uterus as an embryo.

In normal conception, a single ejaculation of semen can contain more than 200 million viable sperm, however, only a few hundred of them.

they reached the released egg in the fallopian tube and have the opportunity to fertilize it. It was once thought that it was impossible to treat men who had a very low sperm count; currently, ICSI fertilization allows a single sperm.

So far they have achieved remarkable results with ICSI, with success even in the case of men with very low sperm count or poor sperm quality. In Brussels, where ICSI has been applied more successfully, up to 70% of injected eggs were fertilized by this method, often with sperm obtained from samples that did not seem to contain enough viable specimens. When transferring the fertilized eggs by ICSI women, pregnancy rates were obtained and birth as high as those registered with IVF (and in some older! Cases).

     Currently these microtechnology have been developed to treat infertility not only men who produce sperm of poor quality, but in patients with complete absence of sperm production due to a blockade or some other testicular disorder (or vasectomy). There are two techniques, microepididimaria sperm aspiration (MESA) and testicular sperm extraction (TESE), currently used regularly to remove sperm from the epididymis (located at the top of the testicles) or a tissue biopsy testicular. Sperm obtained are used to fertilize the egg by ICSI. We have obtained very encouraging results with these techniques, suggesting that men who for various reasons can not ejaculate or whose testicles do not produce sperm can now provide the sperm necessary to fertilize the eggs of their partner.

     Despite the remarkable success of the ICSI, in most centers it is considered that the technique should remain relatively experimental. There is concern that some hereditary diseases - such as cystic fibrosis - associated with male factor infertility can be transmitted to male babies. For this reason, many centers ICSI insist on comprehensive advice and practice of some genetic tests before treatment and follow-up during and after pregnancy.

Infertile couples involved in therapeutic programs ICSI are usually carefully selected (for example, cases of severe defects sperm) and often have a history of failure of the IVE woman should undergo the routine procedure of ovarian stimulation and egg collection while the man must provide a sperm sample (unless they are indicated techniques MESA or TESE).

ICSI step by step:

Pharmacotherapy to stimulate maturation of multiple eggs

GnRH agonists to suppress any other hormonal activity (injections / nasal spray for (usually) two weeks before and after treatment with gonadotropins for 10-14 additional days depending on the response).

E1 gonadotropins to stimulate growth of follicles and cause ovulation

Treatment monitoring to measure the growth of follicles, individualize drug doses, and prevent serious side effects

By transvaginal ultrasound examination (two or three times during a treatment cycle)

Sometimes by quantification of hormones in blood samples

Egg collection, usually under local or general anesthesia lasts 10 to 20 minutes

Guided by transvaginal ultrasound

The eggs are collected from the vagina (32-36 hours after the final hormone injection)

Sperm sample, which is provided on the same day de1a egg collection.

The sample can be obtained naturally or by aspiration in the ILD (dimo (MESA) or removal of the testicles (TESE)

Fertilization

A single sperm is injected into a single egg

The next day the eggs are subjected to a microscopic examination to confirm fertilization

Embryo transfer (usually two to three díasdespués fertilization)

transvaginal transfer embryos no more than three

Placing embryos in the womb

Leftover embryos are usually frozen

Test / monitoring pregnant Preparation and selection of sperm, crucial to the success of ICSI, is carried out by a washing process and classification. These sperm preparation methods allow to obtain only a few viable sperm in a sample not normally considered useful.